This guide will provide users with information on how to get started with the aNCA app.

Step 1: Prepare Dataset

Uploading a dataset

Click Upload File and select the dataset you would like to use. The data can be in .csv, sas7bdat, .xslx, .parquet or .rds format. The best input for the app is data that follows the ADNCA format according to CDISC guidelines. Other formats of data will also work. Alternatively, for exploring the app, Users can use the Dummy dataset that is already uploaded.

Data format

Some variables are mandatory (marked with *), while others are optional. Custom variable names are supported as long as they are correctly mapped during the Data Mapping step.

	Variable	Description	Format
Grouping Identifiers	STUDYID *	Study Identifier	character
	USUBJID *	Unique Subject Identifier	character/numeric
	ATPTREF *	Can be any column, and used to filter data for NCA calculations	any
	TRT01A	Treatment Group Identifier (Actual)	character
	TRT01P	Treatment Group Identifier (Planned)	character
	AGE	Age of Subject	numeric
	RACE	Race of Subject	character
	SEX	Sex of Subject	character
Sample variables	PARAM *	Analyte studied in the sample	character
	PCSPEC *	Specimen Material Type	character
	AVAL *	Analysis Value	numeric
Dose Variables	DOSETRT *	Drug/Treatment compound. When relevant, should match with PARAM	character
	ROUTE *	Route of Administration, either {intravascular} or {extravascular}	character
	DOSEA *	Actual Treatment Dose	numeric
	ADOSEDUR	Actual Treatment Dose	numeric
Time Variables	AFRLT *	Actual Relative Time from Analyte First Dose	numeric
	ARRLT *	Actual Relative Time from Reference Dose	numeric
	NFRLT *	Nominal Relative Time from Analyte First Dose	numeric
	NRRLT *	Nominal Relative Time from Reference Dose	numeric
Time Variables	AVALU	Analysis Value Unit	character
	DOSEU	Treatment Dose Units	character
	RRLTU	Relative Time from Reference Dose Unit	character

Adding Filters

Once your data is uploaded, examine it and then click “Next”. If you want to change the data by adding filters, click the Add Filter button.

Don’t forget to click Submit Filters!

Data Mapping

Click “Next” to get to the Mapping section.

• Map the correct columns in your dataset to the corresponding label.

• For Unit Variables, if your dataset has a defined column for units, select it. Otherwise, by scrolling down, you can select the units for the corresponding columns.

• Click Submit Mapping. You will be automatically directed to the next tab.

Preview

Review the data and ensure that the columns have been mapped correctly, and all your filters have been applied. If there are any problems with your data (duplicates, missing times), application will display a warning message and in certain situations manual edits will be requested. Just follow the instructions.

Step 2: Exploratory Analysis

In the Exploration tab, you will find the different visualization options, including Individual Plots and Mean Plots. Here you can check out your data to see if the profiles and subjects look good.

Step 3: Run NCA

Click on the NCA tab in the menu bar to switch to the next section.

Data selection

1. Settings: Choose the Analyte, Dose Number(s) and/or Specimen(s) you would like to run the NCA on, the extrapolation method and the NCA parameters to calculate. Select additional options including partial AUCs, units, or rule sets to flag the data. In the summary tab, a table summarizes the settings you have selected. You can also download the settings you have created, and upload them again the next time you use the app, which will implement the saved setup.

2. Click Run NCA at the top of the page and check that the data is as expected. You will automatically be redirected to the Results tab.

Step 4: NCA Results

Main Results

The NCA Results tab shows the individual results of the NCA. You can select which parameters you would like to view. Results in red have been flagged as “FALSE” for the rule sets selected, results in purple are flagged as “MISSING”, and results in white are not flagged.

Exclusions and Manual Slopes

Click on the Slopes Information tab to view more detailed information about the slopes selected in the NCA. To add exclusions or manual slope selections, redirect to the Slope Selector tab in the Setup page.

There are two different ways to add exclusions/selections to the slopes.

Via the Buttons

1. For selection and exclusion, click + Exclusion/Selection and input the corresponding information. RANGE refers to the data point number in the slope, which can be seen by hovering over the points in the plots below.
2. To remove, select the sample row and click - Remove selected rows.

Via the Plots

For in-app guidance, you can click the [?]{.help-button} button.

1. For selection, click the first point and then the last point you want included in the slope. The plot should then update and a row should be added to the table. There you can add your reason for selection.
2. For exclusion, double click the point you want to exclude. The plot should update along with the table.
3. To remove, either remove via the table selection, or repeat the same action (i.e. select a new slope, double click the same point).

Applying the exclusions

Once the exclusions/selections have been added, click Run NCA again.

You can check if they have been applied in the Slopes Information page, on the Manual Adjustments tab.

If you do not click Run NCA, the manual slopes will not be added!

Parameter Datasets

In the Parameter Datasets tab you can see and export the ADPP, ADNCA, and PP datasets.

Additional Analysis

In the Additional Analysis tab, you can calculate tissue-matrix ratios using the concentration ratios tab. Input which specimen you would like as the numerator and denominator, and click Submit.

Step 5: TLGs

Currently work in progress. Not all TLGs and features are available.

After performing the analysis, you can prepare reports regarding the analysis, including various tables, listings and graphs.

Order details

Firstly, you will need to prepare an order for TLGs. Table in the Order details tab will display some default TLGs with their metadata. You can add additional reports by clicking the Add TLG button. This will bring up a list of available, defined TLGs. Select the ones you are interested in and click the Add TLGs to Order button. You can also remove unwanted entries by selecting them and clicking Remove TLG.

When you are happy with the selection, click Submit Order Details.

Navigating the TLGs

The application provides three separate tabs for differentiating Tables, Listings and Graphs.

Each tab will show a sidebar on the left of the screen. This will let you navigate each specific entry.

Using the widgets at the top you can specify the number of entries per page and change the pages.

Lastly, the widgets on the right are used for customizing the resulting reports.

Customizing the TLGs

Exact customization options available for any given TLGs will depend on their implementation. Usually, you will be able to specify things like titles and footnotes, axis labels, limits or grouping variables.

Simply provide the desired value in a given widget and the TLG will update in real time.

Leaving a widget empty will induce default behavior of the TLG.

Some fields will provide additional functionalities. For text fields, a special syntax might be used to reference the values or attributes of the data:

• prefacing a column name with ! will reference the label attribute of a given column, e.g. !DOSEU will translate to Treatment Dose Units.
• prefacing a column name with $ will reference the value for a given column, e.g. $DOSEU will translate to mg (for the dummy dataset).

You can always click on the [?]{.help-button} button for help.